Abstract: Objective　To conduct a comparative study on mice models of endometritis induced by Staphylococcus aureus(S. aureus), Escherichia coli (E. coli), and lipopolysaccharide (LPS).Methods Twenty-four female ICR mice were randomly divided into four groups:the control group, the LPS model group, the S. aureus model group, and the E. coli model group, with 6 mice in each group. The inducing agents injected were normal saline, LPS (1 mg/mL), S. aureus (1×109 CFU/mL), and E. coli (1×108 CFU/mL) respectively, at a dose of 50 μL per mouse. The mice were weighed continuously for 7 days. On the 7th day, blood and uterine tissues were collected to detect blood routine and blood biochemical parameters, calculate the uterine index, observe the structure of uterine pathological tissue sections, and determine the changes in inflammatory factors TNF-α and IL-1β in uterine tissues.Results Compared with the control group, the mice in the two bacterial-induced model groups showed a significant increase in white blood cell count, mainly due to the elevation of neutrophils, with significant differences (P<0.05). The uterine index in the S. aureus group increased significantly (P<0.05); the histopathological sections of the three model groups showed varying degrees of endometrial cell damage, loss, and neutrophil exudation; the expression levels of inflammatory factors TNF-α and IL-1β in the S. aureus group and E. coli group increased significantly (P<0.01).Conclusion　This study successfully constructed three mice models of endometritis induced by different factors (S. aureus, E. coli, and LPS), and established the S. aureus-induced mouse endometritis model for the first time. By comparing the blood, tissue, and cytokine aspects, the differences among different induced models were revealed, providing an experimental model for further research on endometritis. 

Key words: endometritis, Escherichia coli, Staphylococcus aureus, LPS, murine animal model

摘要： 目的　探讨了金黄色葡萄球菌、大肠埃希菌及脂多糖诱导的小鼠子宫内膜炎模型的比较研究。方法　实验 将24只ICR雌性小鼠随机分为对照组、脂多糖模型组、金黄色葡萄球菌模型组及大肠埃希菌模型组,每组6只。 注射剂为0.9%氯化钠溶液、脂多糖(1 mg/mL)、金黄色葡萄球菌(1×109 CFU/mL)、大肠埃希菌(1×108 CFU/mL)的 诱导剂50 μL/只,连续7 d称重,第7天采集小鼠的血液及子宫组织,检测血常规数值、血液生化数值;计算子宫指 数;记录子宫病理组织切片结构;检测子宫组织炎性因子TNF-α和IL-1β的变化。结果　与对照组相比,两种细菌 诱导模型组小鼠白细胞数量显著升高,且以中性粒细胞升高为主 (P<0.05);金黄色葡萄球菌组子宫指数显著上升 (P<0.05);3种模型组的组织病理切片表现出不同程度的子宫内膜细胞损伤、缺失,中性粒细胞渗出;金黄色葡萄 球菌组及大肠埃希菌组的炎性因子TNF-α和IL-1β表达显著上升(P<0.01)。结论　本研究成功构建了3种不同 诱导因素(金黄色葡萄球菌、大肠埃希菌、脂多糖)的小鼠子宫内膜炎模型,并首次建立金黄色葡萄球菌诱导小鼠子 宫内膜炎模型。通过对血液、组织及细胞因子等方面的比较,揭示了不同诱导模型间的差异,为子宫内膜炎的进一 步研究提供了实验模型。

关键词: 子宫内膜炎, 大肠埃希菌, 金黄色葡萄球菌, LPS, 小鼠动物模型